MicroRNA signature of leukocytes in the context of chronic systemic inflammation in vascular dementia

Chronic low-level inflammation during the aging process is a key risk factor for activation of resident cells brain innate immune system (microglia and astrocytes). Such leads to development neuroinflammation cognitive impairment which are typical neurodegenerative diseases such as Alzheimers disease, vascular dementia, Parkinson disease etc. Currently, there lack minimally invasive, affordable methods diagnosing age-related drugs that could slow down or prevent their progression. Hence, search new peripheral biomarkers required, both diagnostics monitoring efficiency drug therapy. The option using microRNAs under discussion. Our goal was identify leukocyte microRNA signature in dementia compared with healthy reproductive age, view deficits. We have examined 54 persons from young senile age who were classified into following groups: Vascular Healthy Reproductive age. Expression miRNAs known regulators communications between nervous systems (let-7d, let-7g, miR-21, miR-124, miR-146a, miR-155, miR-342-3p) measured blood leukocytes. decision study leukocytes made, since these responsible functions, and, especially, cytokine production aging. Total RNA isolated by phenol-chloroform technique. expression determined quantitative polymerase chain reaction SYBRGreen. U6 gene small nuclear DNA used reference housekeeping gene. differences groups KruskalWallis test post hoc pairwise comparisons according ConoverInman. As result study, it found microRNA-21 microRNA-342 elderly/senile people, increased when In level miRNA-124 miRNA-342 higher than group. Hence,, microRNA-124 may be informative dementia. However, large-scale studies biomarker potential warranted.